Hexamethylene diisocyanate, 2,4‐toluene diisocyanate, 2,6‐toluene diisocyanate, isophorone diisocyanate and 4,4′‐methylene diphenyl diisocyanate – Determination of hexamethylenediamine, 2,4‐toluenediamine, 2,6‐toluenediamine, isophoronediamine and 4,4′‐methylenedianiline in urine using gas chromatography‐mass spectrometry
Biomonitoring Method – Translation of the German version from 2017
John Cocker1 (Method development)Kate Jones1 (Method development)
Gabriele Leng2 (External verification)
Wolfgang Gries2 (External verification)
Lygia Therese Budnik3 (External verification)
Johannes Müller4 (External verification)
Thomas Göen4 (External verification; Head of the working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft)
Andrea Hartwig5 (Chair of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft)
MAK Commission6
1 Health & Safety Laboratory, Harpur Hill, Buxton SK17 9JN, United Kingdom
2 Currenta GmbH & Co. OHG, CUR-SI-GS – Institute for Biomonitoring, 51368 Leverkusen, Germany
3 Institute for Occupational and Maritime Medicine, Marckmannstraße 129 b, 20539 Hamburg, Germany
4 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Schillerstraße 25 and 29, 91054 Erlangen, Germany
5 Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, Building 50.41, 76131 Karlsruhe, Germany
6 Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Germany
Abstract
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the present biomonitoring method.
The described method allows the simultaneous determination of the metabolites of hexamethylene diisocyanate (HDI), 2,4‐toluene diisocyanate and 2,6‐toluene diisocyanate (TDI), isophorone diisocyanate (IPDI) and methylene diphenyl diisocyanate (MDI) in human urine. After acid hydrolysis the released amines, hexamethylenediamine (HDA), 2,4‐toluenediamine, and 2,6‐toluenediamine (TDA), isophorone diamine (IPDA) and 4,4′‐methylenedianiline (4,4′‐MDA) are extracted from urine, derivatised using heptafluorobutyric anhydride and quantified by NCI‐GC‐MS. The method was extensively validated and the reliability data were confirmed by independent laboratories, which have established and cross‐checked the whole procedure.
