Trichloroethene, 1,1,1‐trichloroethane, 1,1,2,2‐tetrachloroethane and tetrachloroethene – Determination of trichloroacetic acid in urine using headspace‐gas chromatography‐mass spectrometry
Biomonitoring Method – Translation of the German version from 2017
Wolfgang Will1 (Method development)Michael Bader1 (Method development)
Edith Berger‐Preiss2 (External verification)
Thomas Göen3 (Head of the working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft)
Andrea Hartwig4 (Chair of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft)
MAK Commission5
1 BASF SE, Occupational Medicine and Health Protection, GUA/CB, 67056 Ludwigshafen, Germany
2 Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Nikolai-Fuchs-Straße 1, 30625 Hannover, Germany
3 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Schillerstraße 25 and 29, 91054 Erlangen, Germany
4 Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, Building 50.41, 76131 Karlsruhe, Germany
5 Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Germany
Abstract
The described method enables the determination of trichloroacetic acid (TCA) in urine. It is based on the quantitative, thermolytic degradation of TCA into carbon dioxide and chloroform (trichloromethane) in an aqueous solution. The analyte is chloroform, which can be quantified using headspace-gas chromatography and a mass selective detector in the Selected-Ion-Monitoring-mode. For quantitation, a calibration is carried out using standard solutions in pooled urine or water which are processed and analysed in the same way as the samples.
